Objective To collect and summarize the best evidence of bathing for premature infants. Methods We systematically searched the British Medical Journal best practice,UpToDate,the Agency for Healthcare Research and Quality(AHRQ),Registered Nurses’ Association of Ontario(RNAO),National Institute for Health and Care Excellence(NICE),Canadian Medical Association(CMA),Guidelines International Network(GIN),WHO,American College of Physicians(ACP),Joanna Briggs Institute(JBI),Association of Women’s Health,Obstetric and Neonatal Nurses (AWHONN),Cochrane Library,PubMed,CNKI and Wanfang database to collect the literature including best practice,evidence summaries,guidelines,systematic reviews,expert consensuses,etc. The retrieval period was from January,2011 to April,2021. 2 researchers independently evaluated the quality of the literature,combining with the judgment of professionals,to extract the data from the literature meeting the standards. Results A total of 5 articles were incorporated,including 3 guidelines,1 expert consensus,and 1 evidence summary. The best evidence included 27 pieces of evidence in 6 aspects,including bathing assessment,bathing supplies,bathing timing,bath management,rewarming after bath and vernix protection. Conclusion The research summarized the best evidence of bathing for premature infants. The best evidence should be selected based on the environment,conditions and patient’s willingness,in order to reduce complications and adverse events and to ensure bath safety.